Volume: 1 Issue: 1
Year: 2025, Page: 30-33,
Received: July 23, 2024 Accepted: Jan. 10, 2025 Published: March 14, 2025
Aim of the study was to Detect and Quantify High levels of Sucrose content in marketed Pomegranate (Punica granatum) juice by using FT-IR Spectroscopy. The pomegranate fruits were squeezed manually and centrifuged (Make Sorvall, USA, and Model Super T21) at 5000 rpm for 5 min and filtered with muslin cloth to get the clear juice followed by previously weighed sucrose with usage of de-ionised water. Sucrose solutions were prepared by simple stirring, with various concentrations, which represents the sucrose content. Firstly FT-IR (OPASS software) instrument was calibrated by using standard protocol. Compare the obtained spectrum with reference spectrum for wave numbers of 1583 cm-1 to 1589 cm-1, then the % T should be greater than 12% and 2858 cm-1 to 2870 cm-1, the % T should be greater than 18%. After calibration of the FT-IR measure the samples with the scan speed of per sample were 0.2 cm-1. Fourier transformed infrared transmittance spectra were obtained for all samples at room temperature 24±1ºC in the wave number ranging from 4000 cm-1 to 550 cm-1, five scans were performed for each sample and average spectrum was saved for further analysis. ATR-FTIR spectral data was used to predict the adulteration of pomegranate juice by added sucrose. The excess consumption of sucrose present in pomegranate fruit is always associated with obesity, type 2 diabetes, cardiovascular disease, tooth decay, certain cancers, and non-alcoholic fatty liver diseases. It should be regulated by food control authorities, especially fruit drinks marketed to children.
Keywords: Punica granatum, ATR-FTIR, Sucrose content and food safety
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© Vignan’s University, Guntur
Vinod Kumar Kondreddy, Harish Naidu Talluru, Ankanna, Sanjeeva Kumar Avvari. Detection And Quantification of High Levels of Sucrose Content in Marketed Pomegranate ( Punica Granatum ) Juice By Using FT FT-IIR Spectroscopy. Vig J Biotech Phar. 2025;1(1):30-33