Volume: 1 Issue: 1
Year: 2025, Page: 15-22,
Received: July 12, 2024 Accepted: Jan. 10, 2025 Published: April 22, 2025
Animal models play a central role in the biomedical and neurobehavioral research and for producing both experimental data and developing novel ideas. To understand the fundamental characteristics of physiological, behavioral and psychological problems researchers are constantly working to create new animal models. Due to the ethical considerations alternative animal models WERE proposed. Zebrafish (Danio Rerio) now became a popular unique animal model and widely used in neuropharmacology and biomedical research due to their high throughput amenability, screening potential for new therapeutic drugs. The objective of the current investigation was to screen anti-parkinson’s activity and assessing of behavioral alterations in adult zebrafish followed by acute exposure to different pharmacological and herbal compound. Adult zebrafish of 3-4 months old were exposed to different concentrations of test drug (Luffa cylindrica), toxic drug (Paraquat) and the standard drug (Levodopa) for 15 mins. Individually each medication concentration and control were tested. Behavioral activity parameters were performed by using locomotor activity, novel tank diving test and light and dark transition test and catalepsy were recorded for each animal during exposure period. Zebrafish exposed to test drug substance and standard drug displayed behavioral change that were estimated. According to the results, adult zebrafish exposure to test group caused the behavioral changes like recovery of latency to travel from one point to another as compared to toxic group and exhibited significant reversal of the anxious behavior as compared to toxic group. As a result, adult zebrafish can be used as an alternative model for evaluating novel chemical entities on behavioral activity.
Keywords: Zebrafish (DANIO RERIO), Luffa cylindrica leaves, Novel Tank diving Test, Light and Dark Transition Test. Anti-Parkinson’s activity.
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© Vignan’s University, Guntur
I Veena Rani, Archana P, Chandrakanth V, Lavanya A, Rakshith G. Anti-Parkinson’s Activity and Behavioral Assessment of Luffa cylindrica using Zebra Fish Model. Vig J Biotech Phar. 2025;1(1):15-22